Pathways for Biosynthesis of a Bacterial Capsular Polysaccharide

Analysis of a capsular polysaccharide biosynthesis locus of Bacteroides fragilis.

A major clinical manifestation of infection with Bacteroides fragilis is the formation of intra-abdominal abscesses, which are induced by the capsular polysaccharides of this organism. Transposon mutagenesis was used to locate genes involved in the synthesis of capsular polysaccharides. A 24,454-bp region was sequenced and found to contain a 15,379-bp locus (designated wcf) with 16 open reading...

Biosynthesis of the Escherichia coli K5 capsular polysaccharide.

299-304 47 Payne, G., Virji, M. and Saunden, J. R ( I 996) in Abstracts of the Tenth International Pathogenic Neisseria Conference (Zollinger, W.D., Frasch, C.E. and Deal, C.D., eds.), pp. 393-394, National Institutes of Health, Bethesda, MD 48 Parge, H. E.. Forest, K T.. Hickey, M. 1.. Christensen, D. A,. Getzoff, E. D. and Tainer, J. A. (I 995) Nature (London) 378, 32-38 49 Forest, K T. and T...

The biosynthesis of capsular polysaccharide in Aerobacter aerogenes.

RmpA regulation of capsular polysaccharide biosynthesis in Klebsiella pneumoniae CG43.

Sequence analysis of the large virulence plasmid pLVPK in Klebsiella pneumoniae CG43 revealed the presence of another mucoid factor encoding gene rmpA besides rmpA2. Promoter activity measurement indicated that the deletion of rmpA reduced K2 capsular polysaccharide (CPS) biosynthesis, resulting in decreased colony mucoidy and virulence in mice. Introduction of a multicopy plasmid carrying rmpA...

CpsR, a GntR family regulator, transcriptionally regulates capsular polysaccharide biosynthesis and governs bacterial virulence in Streptococcus pneumoniae

Transcriptional regulation of capsule expression is critical for pneumococcal transition from carriage to infection, yet the underlying mechanism remains incompletely understood. Here, we describe the regulation of capsular polysaccharide, one of the most important pneumococcal virulence factor by a GntR family regulator, CpsR. Electrophoretic mobility-shift assays have shown the direct interac...